Hydrogels exhibit a pronounced viscoelastic behavior similar to soft tissues. For this reason, they have been widely used in biomedical research for developing engineered tissues and novel treatments such as wound dressings and drug delivery systems. In this field, hydrogels can be composed of biodegradable natural (e.g. collagen, fibrin, chitosan) and/or synthetic polymers (e.g. PLA, PGA, PLGA). Natural materials can be degraded by naturally occurring enzymes which is an important feature for releasing a drug entrapped in the hydrogel matrix [1] or for allowing the formation of new tissues by seeded cells [2]. Therefore, the precise evaluation of this process is important for designing biomatrices with controlled degradation rates or simply for assessing whether degradation is within the desirable range for the application. In literature, degradation processes have been evaluated by the weight loss of a sample or by its mechanical properties. Since the initial stages of degradation can happen without any loss of weight, the mechanical properties can provide more precise information of the whole degradation process. However, conventional testing technologies such as rheometers and compression mechanical testers are destructive and prevent the re-use of samples for further characterizations (multiple samples are required to study degradation over long periods of time). In this short application note, chitosan-based hydrogels were prepared and immersed with lysozyme solution for the evaluation of their degradation through their viscoelastic properties using ElastoSens™ Bio. The same sample was tested each day during a week in contact with the enzyme.